Common Conjugations

Dyes

 Abbreviation

 Conjugation

 Cy3  Indocarbocyanine 
 Cy5      Indodicarbocyanine
 Variant 1  Variant 2  Cy5.5  Cy7  Cyanine 5.5 Cyanine 7
 FITC  Fluorescein Isothiocyanate
 GFP  Green Fluorescent Protein
 RBITC  Rhodamine B Isothiocyanate
 TRITC  Thetramethylrhodamine Isothiocyanate
 PE  Pycoerythrin
 APC  Allophycocyanin
 PerCP  Peridinin-Chlorophyll-Protein Complex

Other

 Abbreviation

 Conjugation

 KLH  Keyhole Limpet hemocyanin
 BSA  Bovine Serum Albumin
 BTG  Bovine Thyroglobulin
 HRP  Horseradish Peroxidase
 AP  Alkaline Phosphatase

Not abbreviated

 Gold  
 Biotin  
 Streptavidin  

Descriptions

Dye

Absorbance
/Excitation (nm)

Emission (nm)

Emission color

GFP 488 509 Green
FITC 494 520 Green
Cy3 550 570 Yellow
PE 496 578 Yellow
RBITC 540 625 Orange
TRITC 550 600 Orange
APC 650 660 Red
Cy5 650 670 Red
PerCP 482 678 Red
Cy5.5 675 694 Red
Cy7 743 767 Red
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