Home Can total RNA be extracted from paraffin-embedded tissue?

It Is Not Impossible To Extract RNA From It, But It Involves Quite A Lot Difficulty And The RNA Yield Is Very Low That It Is Only Suitable For RT-PCR. The Preserved Conditions Of The Paraffin-Embedded Tissue Are Most Critical. First, If The Tissue Had Been Fixed For More Than 24 Hours, RNA Experienced Modification That Cannot Be Used For RT-PCR Anymore. Second, The Older The Tissue Block Is, The Poorer The RNA Yield Is. Tissue Blocks Stored For More Than 6 Months Are Thus Not Suitable. Third, Only Short-Template RT-PCR (< 400-Bp) Can Be Performed. Fourth, Since Only A Tiny Bit Of Tissue Sample Is Processed, It Is Of Course Hard To Disrupt The Sample Mechanically. In This Case, Proteinase K Should Be Used To Digest The Sample. However, Proteinase K Is Not Provided In The Kit. Fifth, Since Total RNA Extracted Is So Little, RNA Carrier (Like The One Used In Viral RNA Extraction System) Should Be Added To Enhance RNA Recovery. Sixth, Paraffin Needs To Be Removed Before The Tissue Is To Be Disrupted. Immunoport Total RNA Extraction System Is Not Tuned For Processing Paraffin-Embedded Tissues.