or Yeast, Use Blood & Tissue Genomic DNA Extraction System. For Filamentous Fungi And Mushroom, Use Plant Genomic DNA Extraction System.
Yield of genomic DNA extracted is low.
Several Things To Pay Attention To:
1) The Sample May Not Contain Enough Amount Of Genomic DNA. Increase The Sample Amount, Proteinase K, And Buffers Proportionally.
2) Elution Buffer Preheated At 70°C Is Used.
3) DdH2O, Tris-HCl, Or TE Used Should Be Of PH 8.0-9.0.
4) After Adding Elution Buffer Into The Column, Stand The Column For 1-5 Minutes Before Centrifuging To Collect Eluted DNA.