Home Why DNA recovery is lower than expected?

There Are Several Possible Reasons:

1) Do Not Overload The Column With Too Much DNA. Higher Recovery Is Attained When Lower Amount Of DNA Is Loaded. Split Loading High Amount Of DNA Into More Than One Column.

2) If DdH2O Is Used For Elution, Make Sure Than Its PH Is Between 7.0 And 8.5. PH Lower Than 7 Leads To Lower Elution Efficiency.

3) Make Sure That Complete DNA Elution Takes Place By Adding No Less Than 30 Ml Of Elution Solution Onto The Membrane And Letting It Completely Absorbed Into The Membrane Before Centrifugation.

4) Large DNA Fragment Is Eluted Less Readily Than Small DNA Fragment. When The DNA Product Is Larger Than 5-Kb, Use Elution Solution Preheated To 60˚C.