Home Why is TB (terrific broth) not an ideal medium for culturing to prepare plasmids?

It Is Not Recommended To Use Rich Medium Such As TB Or 2X TY For Most Commonly Used Plasmids, Especially High-Copy Number Plasmids. Although Rich Medium Have The Obvious Advantage Of Producing More Bacteria, High Level Of Cell Biomass Reduces The Yield And Quality Of Plasmids. If Rich Medium Must Be Used, Please Reduce The Culture Volume To Match The Suitable Range Of Cell Biomass.

If The Culture Used Too Much, Alkaline Lysis Will Be Inefficient To Lower The Yield Of Extracted Plasmids. Furthermore, The Excessive Viscosity Of The Lysate Will Require Vigorous Mixing To Result In Shearing Of Genomic DNA And Subsequent Contamination Of Extracted Plasmids. High Level Of Protein And Polysaccharide Will Usually Be Carried Out With Plasmids To Result In Bad Quality Of Extracted Plasmids.

To Incubate Bacterial Cells With Rich Medium Overnight (About 16 Hours) Is Not Recommended. Most Of Antibiotics Will Be Ran Out In 8 Hours. The Culture Will Lose The Selection Of The Antibiotic And Reduce The Yield Of Plasmids In The Bacterial Cells.

Incubation With Rich Medium For Long Time, Especially Longer Than 10 Hours, Will Produce Large Amount Of The Dead Cells. Sampling Too Many Dead Cells Will Provide Low Yield And Bad Quality Of Plasmids Extracted.