|Cat No.||XCS 48300/0048|
|Application Notes||Test Label: Biotin labeled primer / FAM probe
Test Format: AmplifyRP® XRT
Internal Control: Yes - Exogenous
Limit of Detection (LOD): 25 pg of genomic DNA; 40 copies of DNA fragments per reaction
Inclusive: Designed to detect all Fusarium oxysporum f. sp. cubense tropical race 4 strains. Strains detected include: NRRL 36114 (VCG 01213), NRRL 54006 (Foc-TR4 reference strain Focll5) and 5 isolates from VCG 01213/16.
|Kit Components||XRT reaction pellets for Foc TR4 (48)
Pre-filled 100 µL PD1 Pellet diluent tubes (48)
AMP1 extraction buffer (55 mL)
Sample bag, mesh (50)
|Features & Benefits||AmplifyRP XRT for Foc TR4 is a rapid DNA amplification and detection platform designed for testing banana root, rhizome, stem, pseudostem, and leaf for Fusarium oxysporum f. sp. cubense tropical race 4 (Fusarium odoratissimum), the causal agent of Panama Disease. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify Foc TR4 DNA and an exogenous DNA control at a single operating temperature (42 °C).
AmplifyRP XRT is a real-time isothermal DNA amplification and detection system that rapidly amplifies small portions of DNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ).
|Booklet||Datasheet | Safety Datasheet|