Common Conjugations
Dyes
Abbreviation |
Conjugation |
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Cy3 |
Indocarbocyanine |
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Cy5
|
Indodicarbocyanine |
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|
Variant 1 Variant 2 |
Cy5.5 Cy7 |
Cyanine 5.5 Cyanine 7 |
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FITC |
Fluorescein Isothiocyanate |
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GFP |
Green Fluorescent Protein |
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RBITC |
Rhodamine B Isothiocyanate |
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TRITC |
Thetramethylrhodamine Isothiocyanate |
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PE |
Pycoerythrin |
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APC |
Allophycocyanin |
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PerCP |
Peridinin-Chlorophyll-Protein Complex |
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Other
Abbreviation |
Conjugation |
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|
KLH |
Keyhole Limpet hemocyanin |
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BSA |
Bovine Serum Albumin |
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BTG |
Bovine Thyroglobulin |
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HRP |
Horseradish Peroxidase |
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|
AP |
Alkaline Phosphatase |
|
Not abbreviated
|
Gold |
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|
Biotin |
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|
Streptavidin |
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Descriptions
Dye |
Absorbance/Excitation (nm) |
Emission (nm) |
Emission color |
|
GFP |
488 |
509 |
Green |
|
FITC |
494 |
520 |
Green |
|
Cy3 |
550 |
570 |
Yellow |
|
PE |
496 |
578 |
Yellow |
|
RBITC |
540 |
625 |
Orange |
|
TRITC |
550 |
600 |
Orange |
|
APC |
650 |
660 |
Red |
|
Cy5 |
650 |
670 |
Red |
|
PerCP |
482 |
678 |
Red |
|
Cy5.5 |
675 |
694 |
Red |
|
Cy7 |
743 |
767 |
Red |
